Wednesday, February 26, 2020

Scholarship Essay Example | Topics and Well Written Essays - 500 words

Scholarship - Essay Example But all that changed when I met my present host family for the first time. There was a warm feeling of kindness and understanding from the moment we first met, and somehow they took me in, and treated me like they would treat their very own daughter. There was this instant feeling of connection, and I knew immediately that I was no longer alone. They helped me with English, and encouraged me to talk more and more in the language, urging me not to be shy, insisting that if I did not make mistakes, I would not learn. Slowly, all the chatter around me began to make sense, and it was a relief to be able to greet the people around me in a a language they understood. The family also encouraged me to take part in their life, and to understand the frank, welcoming American culture. In the course of my stay with them, I have been able to visit various American cities like Boston, New York, Kansas City, San Francisco and Los Angeles in their company. I have seen America like it actually is, and not as I saw it earlier on television, and my respect for this country has grown.

Monday, February 10, 2020

Lab report Example | Topics and Well Written Essays - 1000 words - 5

Lab Report Example In the course of the experiment, the presence of a peptide bond through the application of the method would result to a copper (ii) ion forming violet color in an alkaline solution. The Biuret test in this experiment is used in the determination of the concentration since peptide bonds occur with the same frequency in each amino acid in the peptide. This is possible in this experiment since the absorption at 550 nm is directly proportional to the protein concentration in accordance to Beer-Lambert law, thus enabling the experiment to determine the level of protein concentration in a solution (Janairo et al. 2011). Folin protein assay is a biochemical assay for determination of the total level of protein in a solution. In this experiment, the concentration of protein is manifested in the color change in the sample solution in proportion to the concentration of protein. The method is based on the reaction of Cu+ produced by the oxidation of the peptide bonds (Lowry et al. 1951). For the biuret protein assay, when 3mg protein is used in the experiment, the average absorbance in 550nm is 0.690. For 2.5 mg, the average absorbance is 0,440nm, for 2mg protein, the average absorbance is 0.588nm. When 1.5 mg protein is used, the average absorbance is 0.564 nm, 1mg result to average absorbance of 0.540nm while 0.5ng solution results to an average absorbance of 0.224nm. In tube X the average absorbance is 0.454nm while tube Y which contains approximately half of the volume of X results to an average absorption of 0.232nm. From the experiment, it is evident that the amount of absorption is directly proportionate to the amount of protein concentration in a solution at a standard absorption level of 550nm absorbance. Thus, the biuret protein assay test is a concrete test that can be used in the determination of the peptide bonds and the amount of proteins in a solution (Lipscomb et al. 2006). For